Fwd: Evolution at work:

From: Wade T.Smith (wade_smith@harvard.edu)
Date: Sat Feb 10 2001 - 03:55:57 GMT

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    Evolution at work:

    The tale of a tail - New gene found in fruit flies could impact human
    medicine

    By William J. Cromie
    Gazette Staff

    http://www.hno.harvard.edu/gazette/2001/02.08/01-evolutionatwork.html

    Most people don't think of evolution as very dynamic. If they think of it
    at all, they see it as something that happened in the deep past. But all
    species possess chromosomes, coiled strands of genes in every one of
    their cells, and these genes are constantly changing.

    Take fruit flies, for example. These flying specks of life have been
    buzzing around overripe fruit for some 2 million years, and they are
    still evolving. Or at least their sperm tails are.

    Harvard researchers discovered this fact accidentally, and their
    subsequent investigation of it could lead to a way to detect evolving
    genes in all species, including humans. Besides satisfying scientific
    curiosity, that information could have medical value.

    "Our method might be used to find genes actively evolving in viruses,
    bacteria, and other germs that cause human disease," explains Daniel
    Hartl, Higgins Professor of Biology at Harvard. "This knowledge then
    might be used to find new targets for drugs and other treatments."

    Hartl and his colleagues at Tufts University in Boston and the U.S.
    Department of Agriculture were studying so-called motor proteins in
    fruit-fly sperm tails. These proteins power the swimming motion that
    propels sperm to a docking with female eggs.

    In one type of fly, Drosophilia melanogaster, but not in others, the
    researchers found a gene that carries instructions for making a motor
    that gives this species' sperm extra horsepower. The researchers were not
    looking specifically for such a gene but came across it serendipitously
    during a general investigation of motor proteins.

    Various creatures boast these proteins, which make it possible for them
    to get around in salt water or fresh water, or in a reproductive canal,
    by lashing or spinning threadlike tails. "The tail of D. melanogaster's
    sperm is twice as long as the fly itself," Hartl points out. "Other fruit
    flies may have tails as long as 20 times their body length, intricately
    coiled inside their sex organs until ready for use. We have no idea why
    they grow so long."

    A reversal of roles

    Finding a gene with instructions for making such a protein promised the
    possibility of a view into a corner of evolution that no one had seen
    before. Upon looking into it, the biologists found that D. melanogaster
    evolved a new gene that none of its relatives had. The new gene fused two
    old genes together. A gene region that once carried a code or blueprint
    for making a protein became an on-off switch, and a region that didn't
    code for anything now carries blueprints for making part of a sperm-tail
    motor.

    "It's a beautiful reversal of roles," Hartl comments. "It shows that a
    gene's function is not necessary fixed but can evolve to do something
    quite new."

    Originally, the new gene probably gave fruit flies that had it an
    advantage over those who did not. Sperm motility would have helped them
    in nature's most basic competitive race - reproduction. Now, all males
    have it, so the advantage is less.

    But the story is not over. The gene is still actively undergoing
    evolutionary improvement.

    When a favorable shift like this occurs, it changes the whole
    neighborhood of the chromosome where it happened. Molecular biologists
    can easily see the upheaval with newly available methods of gene mapping.
    For example, if the neighborhood is an old one, researchers see many
    signs of change, i.e., different variations among individual flies. But
    in a newly rebuilt chromosomal neighborhood, little variation exists. The
    latter is what Hartl and his colleagues see in the area of the new
    sperm-tail gene.

    "By carefully studying genetic variation along chromosomes, we're
    reasonably certain we can detect which genes are actively evolving in any
    species, including humans," Hartl says. "That information can be used to
    find these genes in viruses and other organisms that cause disease. Such
    a search might reveal vulnerable sites that we could target with new
    kinds of drugs."

    Human gene activity

    Humans possess a gene that appears to be distantly related to the new
    sperm-tail gene in D. melanogaster. This gene may not be actively
    evolving, but other human genes are. For example, a recently mutated gene
    that has swept through Africa makes people resistant to one type of
    malaria. The gene prevents a parasite called Plasmodium vivax from
    attaching itself to liver and blood cells.

    P. vivax is not a lethal strain of malaria but it makes people very sick.
    "It doesn't kill you, but it makes you wish you were dead," Hartl notes.
    The mutation keeps those who have it from getting sick.

    Malaria kills about 1 million children per year. Most of these deaths are
    due to Plasmodium falciparum, which, like other strains, is transmitted
    by the bite of anopheles mosquitoes. Another comparatively new mutation
    conveys resistance to P. falciparum, but at a high price. It causes
    sickle cell anemia, a painful inherited blood disease that occurs mainly
    in blacks. Sickle cell anemia, however, is not usually fatal.

    These are examples of actively evolving genes in human. A search for such
    evolving genes in malaria parasites might reveal new targets for better
    drugs or even a vaccine.

    Hartl and his team are now mapping the new neighborhood of the fruit fly
    sperm-tail gene in hopes of learning more about how evolution works. They
    are also looking at other genes necessary for the male half of
    reproduction. "They are a particularly interesting group to study," he
    says, "because in all species, including humans, they evolve faster than
    any other group of genes. We'd like to find out why."

    Copyright 2000 President and Fellows of Harvard College

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