From: Derek Gatherer (d.gatherer@vir.gla.ac.uk)
Date: Mon 28 Nov 2005 - 08:50:45 GMT
Before you start fulminating, Ted, I suggest you re-read Hill's
paper. (bottom of p212 and the first paragraph of 213). Then reread
his replay.
At 21:26 27/11/2005, you wrote:
> > From: Derek Gatherer <d.gatherer@vir.gla.ac.uk>
> >
> > Just ask him. I think you'll find the reply interesting.
>
>As I expected, Dr. Hill says the cultures went into clean flasks. I
>refrained from passing on your question because, frankly, it's insulting.
>Of course he used clean flasks! The man is a highly respected scientist.
>He's obviously not going to put his cultures into used flasks. Fortunately
>I didn't have to send him your question as he found it himself on the web
>and graciously passed on his answer to me. Here's his post:
>
>Dear Ted Dace,
>
>Incidentally, I have stumbled on the question by Dr. Gatherer who wrote:
>"What I'd like to ask Dr Hill is, when he splits the unexposed culture, do
>the cells to be exposed to the agent go into the same flask as the previous
>exposure was carried out in? Or is the exposure culture set up in a clean
>flask each time?".
>
>The answer is as follows: At each passage, the cells are split into two
>subpopulations. One of them (a small one) goes into a clean flask with
>growth (nontoxic) medium and the other one (the large one) into a clean
>flask with toxic medium.
>
>Dr. Gatherer can send next questions directly to me on my address
>hill@infobiogen.fr. I am not used to forum discussions on the Internet
>though I read my email.
>
>Sincerely yours,
>Dr. Miroslav Hill
>
> > At 22:51 22/11/2005, you wrote:
> > > > From: Derek Gatherer <d.gatherer@vir.gla.ac.uk>
> > > >
> > > > What I'd like to ask Dr Hill is, when he splits the unexposed
> > > > culture, do the cells to be exposed to the agent go into the same
> > > > flask as the previous exposure was carried out in? Or is the
> > > > exposure culture set up in a clean flask each time?
> > >
> > >Your question seems to be irrelevant. Even if Hill failed to switch out
>the
> > >flask into which he placed the exposed culture (a doubtful prospect) how
> > >would this result in contanimation of unexposed cultures?
> >
>
>
>
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For information about the journal and the list (e.g. unsubscribing)
see: http://www.cpm.mmu.ac.uk/jom-emit
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