From: Dace (firstname.lastname@example.org)
Date: Sun 27 Nov 2005 - 21:26:54 GMT
> From: Derek Gatherer <email@example.com>
> Just ask him. I think you'll find the reply interesting.
As I expected, Dr. Hill says the cultures went into clean flasks. I
refrained from passing on your question because, frankly, it's insulting.
Of course he used clean flasks! The man is a highly respected scientist.
He's obviously not going to put his cultures into used flasks. Fortunately
I didn't have to send him your question as he found it himself on the web
and graciously passed on his answer to me. Here's his post:
Dear Ted Dace,
Incidentally, I have stumbled on the question by Dr. Gatherer who wrote:
"What I'd like to ask Dr Hill is, when he splits the unexposed culture, do the cells to be exposed to the agent go into the same flask as the previous exposure was carried out in? Or is the exposure culture set up in a clean flask each time?".
The answer is as follows: At each passage, the cells are split into two
subpopulations. One of them (a small one) goes into a clean flask with
growth (nontoxic) medium and the other one (the large one) into a clean
flask with toxic medium.
Dr. Gatherer can send next questions directly to me on my address
firstname.lastname@example.org. I am not used to forum discussions on the Internet
though I read my email.
Dr. Miroslav Hill
> At 22:51 22/11/2005, you wrote:
> > > From: Derek Gatherer <email@example.com>
> > >
> > > What I'd like to ask Dr Hill is, when he splits the unexposed
> > > culture, do the cells to be exposed to the agent go into the same
> > > flask as the previous exposure was carried out in? Or is the
> > > exposure culture set up in a clean flask each time?
> >Your question seems to be irrelevant. Even if Hill failed to switch out
> >flask into which he placed the exposed culture (a doubtful prospect) how
> >would this result in contanimation of unexposed cultures?
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